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M9470141.TXT
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1994-07-02
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Document 0141
DOCN M9470141
TI Effect of human immunodeficiency virus type 1 (HIV-1) monocyte-derived
macrophages infection on the manganous superoxide dismutase gene
expression.
DT 9409
AU Raoul H; Mabondzo A; Le Naour R; Dormont D; Laboratoire de
Neuropathologie experimentale et Neurovirologie,; CRSSA/DSV/DPTE,
Commissariat a l'Energie Atomique, Fontenay aux; Roses, France.
SO Chem Biol Interact. 1994 Jun;91(2-3):123-31. Unique Identifier :
AIDSLINE MED/94251838
AB Clinical and biological features indicate that a dysregulation of
microbicidal activity occurs in the cells of mononuclear phagocytic
lineage of HIV-1-infected patients. Thus, the regulation of MnSOD gene
expression has been investigated during the 10 h following in vitro HIV
macrophage infection. As previously reported, in HIV-1 LAI-infected
macrophages a high expression of the MnSOD gene is observed 2 and 4 h
after infection. These results are confirmed when cells are infected
with three macrophage-tropic strains HIV-1, DAS, PAR and Bal. Moreover,
the detection of the MnSOD gene expression in the macrophage cultures is
associated with the cellular tropism of the viral strains used. The
binding of recombinant GP160 by itself is not sufficient to induce MnSOD
expression. In fact, the same MnSOD gene induction was obtained with the
heat inactivated viral isolates, indicating that these phenomena are due
to the viral entry. On the other hand, phagocytosis of latex beads
triggers a high expression of the MnSOD gene in macrophages, showing
that phagocytosis of HIV may be sufficient to induce the expression of
that gene. Taken together, these results indicate that the MnSOD gene
expression observed within 10 h following infection of macrophages is
mainly related to membrane biophysical unspecific modifications.
DE Blotting, Northern Cells, Cultured Endocytosis *Gene Expression
Regulation, Enzymologic Gene Products, env/METABOLISM Human
HIV-1/*PHYSIOLOGY Macrophages/ENZYMOLOGY/*MICROBIOLOGY Monocytes
Protein Precursors/METABOLISM Recombinant Proteins/METABOLISM
Superoxide Dismutase/BIOSYNTHESIS/*GENETICS Support, Non-U.S. Gov't
Transcription, Genetic JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).